α-globulin-rich rice cultivar, low glutelin content-1 (LGC-1), decreases serum cholesterol concentration in exogenously hypercholesterolemic rats.

BACKGROUND: Rice α-globulin has been reported to have serum cholesterol-lowering activity in rats. However, it is still unclear whether α-globulin exerts this effect when taken as one of the dietary components. In the present study, we investigated the effect of two cultivars of rice, low glutelin content (LGC)-1 and LGC-Jun, on reducing serum cholesterol in exogenously hypercholesterolemic (ExHC) rats. LGC-1 is enriched in α-globulin (10.6 mg g-1 rice flour, which is an approximately 1.5 times higher α-globulin content than in Koshihikari a predominant rice cultivar in Japan), whereas LGC-Jun is a globulin-negative cultivar. METHODS: ExHC rats, the model strain of diet-induced hypercholesterolemia, were fed 50% LGC-1 or LGC-Jun and 0.5% cholesterol-containing diets for 2 weeks, followed by measurement of cholesterol metabolism parameters in serum and tissues. RESULTS: Serum cholesterol and non-high-density lipoprotein cholesterol levels were significantly lower in the LGC-1 group compared to the LGC-Jun group. Cholesterol intestinal absorption markers, hepatic and serum levels of campesterol and ß-sitosterol, and lymphatic cholesterol transport were not different between the two groups. Levels of 7α-hydroxycholesterol, an intermediate of bile acid synthesis, showed a downward trend in the livers of rats that were fed LGC-1 (P = 0.098). There was a significant decrease in the hepatic mRNA expression of Cyp7a1 (a synthetic enzyme for 7α-hydroxycholesterol) in the LGC-1 group compared to the LGC-Jun group. CONCLUSION: Dietary LGC-1 significantly decreased serum cholesterol levels in ExHC rats. The possible mechanism for the cholesterol-lowering activity of LGC-1 is partial inhibition of bile acid and cholesterol synthesis in the liver. © 2021 Society of Chemical Industry.

Electrophysiological responses from intrinsically photosensitive retinal ganglion cells are diminished in glaucoma patients / Disminución de las respuestas electrofisiológicas de las células ganglionares de la retina intrínsecamente fotosensibles en los pacientes con glaucoma

Purpose: To record electroretinograms (ERGs) from intrinsically photosensitive retinal ganglion cells (ipRGCs) of glaucoma patients. Methods: ERGs were recorded in 10 normal subjects and 15 patients with glaucoma. The ERG illumination system was built to achieve receptor-silent substitution, and comprised an optical diffuser and four-in-one light-emitting diodes. Results: The ERG recordings of ipRGC from normal subjects showed an «on» response and an «off» response. The mean (± SD) implicit time for the on and off responses in normal subjects was 103.0 ± 24.9 and 337.9 ± 45.8ms, respectively, with corresponding amplitudes of 7.7 ± 2.8 and 7.3 ± 3.4μV, respectively. In glaucoma patients, the implicit time of the on and off responses was 135.0±28.9 and 368.2 ± 17.3ms, respectively. The corresponding amplitudes of the on and off responses in these patients were 0.47±0.18 and 0.66 ± 0.32μV, respectively. Conclusions: The results demonstrate successful ERG recording of ipRGCs from advanced glaucoma patients, with marked reductions in amplitude, although not implicit time, compared with normal subjects (AU)

Objetivo: Registrar los electrorretinogramas (ERG) de las células ganglionares de la retina, intrínsecamente fotosensibles (ipRGCs) de los pacientes con glaucoma. Métodos: Se registraron los ERG de diez sujetos normales, y de quince pacientes con glaucoma. El sistema de iluminación del ERG se preparó para lograr la sustitución de los receptores silentes, incluyendo un difusor óptico y un sistema de cuatro-en-uno diodos emisores de luz. Resultados: Los registros ERG de las ipRGC en los sujetos normales reflejaron una respuesta «on» y una respuesta «off». El tiempo implícito medio (± DE) para las respuestas «on» y «off» en los sujetos normales fue de 103,0 ± 24,9 y 337,9 ± 45,8ms, respectivamente, con amplitudes correspondientes de 7,7 ± 2,8 y 7,3 ± 3,4μV, respectivamente. En los pacientes con glaucoma, el tiempo implícito de las respuestas «on» y «off» fue de 135,0 ± 28,9 y 368,2 ± 17,3ms, respectivamente. Las amplitudes correspondientes de las respuestas «on» y «off» en dichos pacientes fueron de 0,47 ± 0,18 y 0,66 ± 0,32μV, respectivamente. Conclusiones: Los resultados demuestran unos registros exitosos de ERG de los ipRGCs en pacientes con glaucoma avanzado, con unas reducciones marcadas en cuanto a amplitud, aunque no en lo referente a tiempo implícito, en comparación a los sujetos normales (AU)

Effects of the light-dark cycle on diurnal rhythms of diet-induced thermogenesis in humans.

This study aimed to clarify the effect of light exposure during the daytime and nighttime on diet-induced thermogenesis (DIT), which is one kind of energy expenditure, and the contribution of autonomic nervous activities (ANA) to the mechanism behind such effects. We found that the light-dark cycle significantly induced a diurnal rhythm of DIT, with afternoon levels tending to be higher than nighttime levels. By contrast, no such rhythms were observed under constant light or dark conditions. There were also no significant differences in ANA between the light conditions. These findings demonstrate that a diminished light-dark cycle leads to disruption of the diurnal rhythm of metabolism and so the retention of ordinary light-dark cycles may be recommended for health maintenance.

A novel intrinsic electroretinogram response in isolated mouse retina.

Since the discovery of intrinsic photosensitive retinal ganglion cell (ipRGC) was reported in 2002, many features specific to this cell type have been described. However, scare information is available on the retinographic components directly reflecting ipRGC activity. In this study, we identified the electroretinogram (microERG) that reflects the photoresponses by ipRGCs in ex vivo preparations of the mouse retina, in which classical photoreceptors (cones and rods) were ablated mechanically and photochemically. MicroERG consisted of three components: a large transient ON response, a small and lazy hump 19s after the onset of the light, and a large transient OFF response. A complete microERG recording required at least 30s of light exposure. MicroERG showed the highest spectral photosensitivity at 478nm. This wavelength corresponds to the peak wavelength in the ipRGCs' photosensitive curve. The psychophysical test using a blue light-emitting diode (LED) light (470nm) revealed that the absolute threshold illuminance for microERG was greater than 12.26 log photons/s/cm2 in both ON and OFF responses, whereas microERG was not adapted for dark. The amplitude of microERG increased linearly with irradiance. The sensitivity of temporal frequency was high in microERG (at least 100Hz), as suggested by the study on melatonin suppression by flickering light in human subjects (Zelter et al., 2014). Melatonin secretion was suppressed by light via ipRGCs and the suprachiasmatic nucleus. These properties of the photoresponse indicate that microERG may reflect the functions of ipRGC as a luminance detector in the mouse retina.

Electrophysiological responses from intrinsically photosensitive retinal ganglion cells are diminished in glaucoma patients.

PURPOSE: To record electroretinograms (ERGs) from intrinsically photosensitive retinal ganglion cells (ipRGCs) of glaucoma patients. METHODS: ERGs were recorded in 10 normal subjects and 15 patients with glaucoma. The ERG illumination system was built to achieve receptor-silent substitution, and comprised an optical diffuser and four-in-one light-emitting diodes. RESULTS: The ERG recordings of ipRGC from normal subjects showed an "on" response and an "off" response. The mean (±SD) implicit time for the on and off responses in normal subjects was 103.0±24.9 and 337.9±45.8ms, respectively, with corresponding amplitudes of 7.7±2.8 and 7.3±3.4µV, respectively. In glaucoma patients, the implicit time of the on and off responses was 135.0±28.9 and 368.2±17.3ms, respectively. The corresponding amplitudes of the on and off responses in these patients were 0.47±0.18 and 0.66±0.32µV, respectively. CONCLUSIONS: The results demonstrate successful ERG recording of ipRGCs from advanced glaucoma patients, with marked reductions in amplitude, although not implicit time, compared with normal subjects.

Effects of tryptophan-rich breakfast and light exposure during the daytime on melatonin secretion at night.

BACKGROUND: The purpose of the present study is to investigate effects of tryptophan intake and light exposure on melatonin secretion and sleep by modifying tryptophan ingestion at breakfast and light exposure during the daytime, and measuring sleep quality (by using actigraphy and the OSA sleep inventory) and melatonin secretion at night. METHODS: Thirty three male University students (mean ± SD age: 22 ± 3.1 years) completed the experiments lasting 5 days and 4 nights. The subjects were randomly divided into four groups: Poor*Dim (n = 10), meaning a tryptophan-poor breakfast (55 mg/meal) in the morning and dim light environment (<50 lx) during the daytime; Rich*Dim (n = 7), tryptophan-rich breakfast (476 mg/meal) and dim light environment; Poor*Bright (n = 9), tryptophan-poor breakfast and bright light environment (>5,000 lx); and Rich*Bright (n = 7), tryptophan-rich breakfast and bright light. RESULTS: Saliva melatonin concentrations on the fourth day were significantly lower than on the first day in the Poor*Dim group, whereas they were higher on the fourth day in the Rich*Bright group. Creatinine-adjusted melatonin in urine showed the same direction as saliva melatonin concentrations. These results indicate that the combination of a tryptophan-rich breakfast and bright light exposure during the daytime could promote melatonin secretion at night; further, the observations that the Rich*Bright group had higher melatonin concentrations than the Rich*Dim group, despite no significant differences being observed between the Poor*Dim and Rich*Dim groups nor the Poor*Bright and Rich*Bright groups, suggest that bright light exposure in the daytime is an important contributor to raised melatonin levels in the evening. CONCLUSIONS: This study is the first to report the quantitative effects of changed tryptophan intake at breakfast combined with daytime light exposure on melatonin secretion and sleep quality. Evening saliva melatonin secretion changed significantly and indicated that a tryptophan-rich breakfast and bright light exposure during the daytime promoted melatonin secretion at this time.

Daily rhythms of the sleep-wake cycle.

The amount and timing of sleep and sleep architecture (sleep stages) are determined by several factors, important among which are the environment, circadian rhythms and time awake. Separating the roles played by these factors requires specific protocols, including the constant routine and altered sleep-wake schedules. Results from such protocols have led to the discovery of the factors that determine the amounts and distribution of slow wave and rapid eye movement sleep as well as to the development of models to determine the amount and timing of sleep. One successful model postulates two processes. The first is process S, which is due to sleep pressure (and increases with time awake) and is attributed to a 'sleep homeostat'. Process S reverses during slow wave sleep (when it is called process S'). The second is process C, which shows a daily rhythm that is parallel to the rhythm of core temperature. Processes S and C combine approximately additively to determine the times of sleep onset and waking. The model has proved useful in describing normal sleep in adults. Current work aims to identify the detailed nature of processes S and C. The model can also be applied to circumstances when the sleep-wake cycle is different from the norm in some way. These circumstances include: those who are poor sleepers or short sleepers; the role an individual's chronotype (a measure of how the timing of the individual's preferred sleep-wake cycle compares with the average for a population); and changes in the sleep-wake cycle with age, particularly in adolescence and aging, since individuals tend to prefer to go to sleep later during adolescence and earlier in old age. In all circumstances, the evidence that sleep times and architecture are altered and the possible causes of these changes (including altered S, S' and C processes) are examined.

Distinct responses of cones and melanopsin-expressing retinal ganglion cells in the human electroretinogram.

BACKGROUND: The discovery of the novel photoreceptor, melanopsin-expressing retinal ganglion cells (mRGCs), has raised researchers' interest in photoreceptive tasks performed by the mRGC, especially in non-image-forming visual functions. In a prior study, we investigated the mRGC response to light stimuli independent of rods and cones with the four-primary illumination system, which modulates stimulus levels to the mRGC and cones independently, and mRGC baseline responses were recorded in the electroretinogram (ERG). METHODS: In the present study, we used the same illumination system to compare independent responses of the mRGC and cones in five subjects (mean ± SD age, 23.0 ± 1.7 years). The ERG waveforms were examined as direct measurements of responses of the mRGCs and cones to stimulation (250 msec). Implicit times (the time taken to peaks) and peak values from 30 stimuli given to each subject were analyzed. RESULTS: Two distinct positive peaks appeared in the mRGC response, approximately 80 msec after the onset of the stimuli and 30 msec after their offset, while no such peaks appeared in the cone response. The response to the mRGC stimulus was significantly higher than that to the cone stimulus at approximately 80 msec (P < 0.05) and tended to be higher than the cone stimulus at approximately 280 msec (P = 0.08). CONCLUSIONS: Implicit time of the first peak was much longer than that to the b-wave and this delay might reflect mRGC's sluggish responses. This is the first report of amplitudes and implicit time in the ERG from the response of the mRGC that is independent of rods and cones, and obtained using the four-primary illumination system.

The ERG responses to light stimuli of melanopsin-expressing retinal ganglion cells that are independent of rods and cones.

The mechanisms by which melanopsin-expressing retinal ganglion cells (mRGCs) regulate circadian rhythms in humans have not been established. To understand mRGC characteristics and their role independent of effects due to the rods and cones, mRGC responses should be induced or measured independent of cone and rod responses. In the present study, we obtained results from light stimuli which differentially induce only the mRGC response by using a receptor-silent substitution technique. The mRGCs responded linearly to contrast changes of light stimuli, whereas they showed complicated responses to frequency changes with regard to the latency of response time. These results suggest that mRGC behavior is not a simple response to the various frequencies found in solar light but may be related to intrinsic neural circuits with feedback connections in the mRGC pathway. The results in this study also demonstrated that the test stimuli affected only the mRGC response and that this could be successfully detected by using the electroretinogram (ERG).